已知下丘脑及垂体中、前叶有高浓度的β-内啡肽(β-endorphin,β-EP),但它是否参与垂体前叶激素释放的调节却未尽明了。我们采用第三脑室内注射β-EP的抗血清,能较特异地阻断β-EP的作用,以探讨中枢内源性β-EP对ACTH和催乳素释放的影响。 实验用成年Sprague-Dawley雄性大鼠,体重270±10克。每只动物埋植第三脑室导管一周后,于实验前一天通过颈外静脉预置硅橡胶导管至右心房水平,以便翌日正式实验时 It is known that there is a high concentration of β-endorphin (β-EP) in the hypothalamus and pituitary, but whether it is involved in the regulation of anterior pituitary hormone release is unknown. We use the third intraventricular injection of β-EP antiserum, can more specifically block the role of β-EP, to explore the central endogenous β-EP on ACTH and prolactin release. Adult Sprague-Dawley male rats, weighing 270 ± 10 grams, were used. One week after the third ventricle catheter was implanted in each animal, the silicone rubber catheter was placed through the external jugular vein to the level of the right atrium on the day prior to the experiment so as to be formally tested on the following day