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目的采用HPLC法测定大鼠血浆中莽草酸的含量。方法大鼠血浆样品经甲醇沉淀蛋白后,采用HPLC法测定血浆中莽草酸的含量。色谱柱为Wonda Cract ODS-2(250 mm×4.6 mm,5μm),流动相为甲醇(3 mmol·L~(-1)四庚基溴化铵)-5mmol·L~(-1)磷酸(用1 mol·L~(-1)Na OH调p H5.8)(60∶40),检测波长220 nm,流速0.8 m L·min~(-1),柱温35℃。结果血浆中莽草酸的线性范围为0.525~10.50μg·m L~(-1)(r=0.9998),平均回收率为97.7%~101.6%,日内、日间RSD均<15%。结论所用方法简便、灵敏、结果准确,适用于测定生物样品中莽草酸的浓度。
Objective To determine the content of shikimic acid in rat plasma by HPLC. Methods Plasma samples from rat plasma were precipitated with methanol and the contents of shikimic acid in plasma were determined by HPLC. The mobile phase consisted of Wonda Cract ODS-2 (250 mm × 4.6 mm, 5 μm) and mobile phase consisted of methanol (3 mmol·L -1 tetraheptylammonium bromide) -5 mmol·L -1 phosphoric acid The detection wavelength was set at 220 nm and the flow rate was 0.8 m L · min -1 with 1 mol·L -1 NaOH (pH 7.5). The column temperature was 35 ℃. Results The linear range of shikimic acid in plasma was 0.525-10.50μg · m L -1 (r = 0.9998). The average recovery was 97.7% -101.6%. The intra-day and inter-day RSD were less than 15%. Conclusion The method is simple, sensitive and accurate. It is suitable for the determination of shikimic acid in biological samples.