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目的探讨三七皂苷R1对UV诱导的人皮肤成纤维细胞的保护作用及其相关分子机制。方法采用不同剂量UV照射原代培养的人皮肤成纤维细胞,同时加入三七皂苷R1干预处理,倒置相差显微镜观察细胞损伤的形态学变化,并用MTT法检测其增殖活性,比色法检测成纤维细胞培养上清中羟脯氨酸的含量,ELISA法检测成纤维细胞MMP-1蛋白水平。结果 UVB、UVA辐射成纤维细胞后,细胞损伤程度呈剂量依赖性,加入三七皂苷R1后,体外培养成纤维细胞未出现明显的数量上和形态学改变;三七皂苷R1浓度为5,20μg.mL-1时经UV照射的FB细胞增殖活性增加;三七皂苷R1浓度为5,20μg.mL-1时抑制MMP-1的分泌。结论 UVB、UVA辐射对皮肤成纤维细胞具有明显的损伤作用,而三七皂苷R1对其具有一定的保护作用。
Objective To investigate the protective effect of notoginsenoside R1 on UV-induced human dermal fibroblasts and its related molecular mechanisms. Methods Human dermal fibroblasts cultured in primary culture were exposed to different doses of UV light. Panax notoginseng saponins R1 was added to treat human skin fibroblasts. Morphological changes of cell injury were observed by inverted phase contrast microscope. MTT assay was used to detect the proliferation of fibroblasts. The content of hydroxyproline in the cell culture supernatant and the level of MMP-1 protein in fibroblasts were detected by ELISA. Results After UVB and UVA irradiation, the degree of cell damage was dose-dependent. After addition of notoginsenoside R1, there was no obvious quantitative and morphological changes in fibroblasts cultured in vitro. The concentration of notoginsenoside R1 was 5 and 20μg .The proliferation activity of UV-irradiated FB cells was increased when mL-1 was added; the secretion of MMP-1 was inhibited when the concentration of notoginsenoside R1 was 5 and 20μg.mL-1. Conclusion UVB and UVA radiation have a significant damage on skin fibroblasts, while notoginsenoside R1 has certain protective effect on it.