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目的:探讨血清谷胱甘肽S-转移酶（GST）在复合因素所致免疫性肝损伤中的变化规律及意义。方法:用卡介苗（BCG）和脂多糖（LPS）经尾静脉注射制造小鼠肝损伤模型,在不同时点测定血清丙氨酸转氨酶（ALT）和GST活性,观察肝脏病理学改变,并分析血清ALT、肝组织学改变与血清GST间的相关性。结果:LPS注射4h后,血清GST和ALT活性均开始显著性升高,血清GST于6h达高峰,10h恢复正常,血清ALT活性于8h达高峰,随后缓慢下降,15h仍高于正常。血清GST与ALT活性变化呈正相关,与肝组织病理学改变无相关关系。结论:BCG和LPS导致的小鼠免疫性肝损伤模型中,血清CST是反映肝功能变化的良好生化指标,较血清ALT灵敏。
Objective: To investigate the regularity and significance of serum glutathione S-transferase (GST) in immune-induced liver injury caused by multiple factors. Methods: The model of hepatic injury in mice was made by tail vein injection of BCG and lipopolysaccharide (LPS). Serum alanine aminotransferase (ALT) and GST activity were measured at different time points. The pathological changes of liver were observed, ALT, liver histological changes and serum GST correlation. RESULTS: After 4h injection of LPS, serum GST and ALT activity began to increase significantly. Serum GST peaked at 6h and returned to normal at 10h. Serum ALT activity peaked at 8h, then decreased slowly and remained higher than normal at 15h. There was a positive correlation between serum GST and ALT activity and no correlation with liver histopathology. CONCLUSION: Serum CST is a good biochemical index reflecting the change of liver function in mice with immunological liver injury induced by BCG and LPS, which is more sensitive than serum ALT.