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作者们介绍了喉乳头状瘤自体疫苗治疗的材料及方法: 一、疫苗制备: (一)组织准备: 1.从病人喉部取下新鲜的乳头状瘤,立即放入4℃的消毒生理盐水中保存,以备当天制备疫苗用。 2.以剪刀剪除与肿瘤粘连的正常组织,将瘤组织绞碎后放入消毒的组织匀浆器内,以消毒生理盐水洗涤之,并使其匀浆化,将匀浆倒入预先称好的15ml锥形离心管内,以1500xg离心沉淀。弃去上清液,将0.1％的甲醛10ml加入沉淀物中,再将沉淀物重新混匀后放入37℃的环
The authors introduced materials and methods for the treatment of laryngeal papillomavirus self-vaccine: First, the vaccine preparation: (a) tissue preparation: 1. Remove the fresh papilloma from the throat of the patient, immediately placed in sterile water at 4 ℃ saline In the preparation for the day to prepare vaccine. 2. With scissors to cut off the normal tissue adhesion with the tumor, the tumor tissue after minced into sterile tissue homogenizer to disinfect saline wash, and make it homogenized, the homogenate into the pre-weighed In a 15 ml conical centrifuge tube and centrifuged at 1500 xg. The supernatant was discarded, 10 ml of 0.1% formaldehyde was added to the precipitate, and the precipitate was re-mixed and placed in a 37 ° C loop