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目的:探讨三氧化二砷对HL-60的诱导凋亡作用。方法:琼脂糖电泳检测DNA条带;Hoecst 33258染色后荧光显微镜观察细胞核的变化。用流式细胞仪检测细胞凋亡率。结果:10μmol/L三氧化二砷处理HL-60细胞24~48 h可见到DNA出现梯形条带;处理24~48 h HL-60细胞核呈现细胞核浓缩和核碎裂现象。流式细胞仪测定12、24、和48 h的细胞凋亡率分别为(8.5±2.1)%、(12.8±3.4)%及(21.4±5.8)%,而培养48 h的HL-60细胞自然凋亡率仅为(1.5±0.5)%(P<0.05)。结论:三氧化二砷可诱导HL-60细胞凋亡。
Objective: To investigate the apoptosis induced by arsenic trioxide on HL-60. Methods: The DNA bands were detected by agarose gel electrophoresis. The changes of nucleus were observed by Hoecst 33258 staining. Flow cytometry was used to detect apoptosis rate. Results: DNA ladder appeared in HL-60 cells treated with 10μmol / L arsenic trioxide for 24-48 h. Nucleus concentration and nuclear fragmentation were observed in HL-60 cells treated with 10μmol / L arsenic trioxide for 24-48 hours. The apoptotic rates of HL-60 cells cultured for 48 h were (8.5 ± 2.1)%, (12.8 ± 3.4)% and (21.4 ± 5.8)% respectively when measured by flow cytometry at 12, 24 and 48 h The apoptosis rate was only (1.5 ± 0.5)% (P <0.05). Conclusion: Arsenic trioxide can induce HL-60 cell apoptosis.