利用不同剂量髓鞘少突胶质细胞糖蛋白(Myelin oligodendrocytes glycoprotein,MOG34-56)免疫诱导制备食蟹猴(Macaca fascicularis)实验性自身免疫性脑脊髓炎模型(Experimental autoimmune encephalomyelitis,EAE)。选择4岁左右雌性食蟹猴,分高、低2个剂量组,利用合成的MOG34-56与完全弗氏佐剂(Complete Freund’s adjuvant,CFA)混合制备成乳剂,经腋窝和腹股沟皮下多点免疫注射的方法,制备EAE食蟹猴模型,从行为学、核磁共振成像(Magnetic Resonance Imaging,MRI))和病理学等多方面进行评估。结果显示利用不同剂量MOG34-56在完全弗氏佐剂条件下通过皮下多点免疫方法能成功诱导出不同病程的EAE食蟹猴模型,且诱导发病率达100%。表现为行为学异常,体重下降,神经系统显示功能障碍,MRI影像及解剖病理观察均有明显病变,且高剂量诱导组症状较低剂量组严重。 Experimental autoimmune encephalomyelitis (EAE) was induced by immunization with different doses of myelin oligodendrocytes glycoprotein (MOG34-56). Four-year-old female cynomolgus monkeys were selected and divided into two groups: high and low dose groups. MOG34-56 and Complete Freund’s adjuvant (CFA) were used to prepare the emulsion. Multi-point immunostimulation was performed subcutaneously in the armpits and groin. Injection method, prepared EAE cynomolgus monkey model, from the behavior, magnetic resonance imaging (Magnetic Resonance Imaging, MRI)) and pathology and other aspects of assessment. The results showed that different doses of MOG34-56 under complete Freund’s adjuvant can successfully induce EAE cynomolgus monkey model with different course through subcutaneous multi-point immunization, and the incidence of induction is up to 100%. Manifested as behavioral abnormalities, weight loss, nervous system dysfunction, MRI images and anatomical pathology were significantly lesion, and high-dose induction group symptoms lower dose group.