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OBJECTIVE To develop a simple high performance liquid chromatographic method for the determination of biphenyl dimethyl dicarboxylate in rat plasma. METHODS To use the methods of high performance liquid chromatography.The method used a smaller sample volume(0.1mL) and added 2.5volumes of acetonitrile for the determination of biphenyl dimethyl dicarboxylate. RESULTS The detection limit in rat plasma was 50?μg/L.The mean percentage recovery of the drug in the concentration range of 0.05～5?mg/L was 94.13% while the mean of the inter day coefficient of variation of the same concentration range was 2.39%. CONCLUSION The method needn’t internalstandard and the involved course of extraction.
OBJECTIVE To develop a simple high performance liquid chromatographic method for the determination of biphenyl dimethyl dicarboxylate in rat plasma. METHODS To use the methods of high performance liquid chromatography. The method used in smaller sample volume (0.1 mL) and added 2.5 volumes of acetonitrile the determination of biphenyl dimethyl dicarboxylate. RESULTS The detection limit in rat plasma was 50 μg / L. The mean percentage recovery of the drug in the concentration range of 0.05-5 mg / L was 94.13% while the mean of the inter day coefficient of variation of the same concentration range was 2.39%. CONCLUSION The method need not internalstandard and the involved course of extraction.