论文部分内容阅读
目的 研究糖尿病 (DM )大鼠血清一氧化氮 (NO)与早期骨代谢改变的关系。方法2 0只SD大鼠分为 2组 ,一组以链脲佐菌素诱导建立糖尿病 (STZ DM )大鼠模型 ,另一为正常对照组 ,测定 2组大鼠的空腹血糖 (FBG)、HbA1c、血清胰岛素、全身、股骨和腰椎骨密度 (BMD)、骨代谢相关指标〔血清钙、骨钙素、降钙素、甲状旁腺素 (PTH)、维生素D3 及尿吡啶酚 /肌酐比〕和血清NO水平。结果 STZ DM大鼠与正常对照组相比 ,血清NO水平显著升高〔(5 1.3± 11.9vs 38.1± 12 .0 )μmol/L ,P <0 .0 1〕 ;全身、股骨和腰椎的BMD显著降低〔(0 .15± 0 .0 7vs 0 .2 1± 0 .0 2 ) g/cm2 ,P<0 .0 1;(0 .16± 0 .0 2vs 0 .19± 0 .0 3) g/cm2 ,P <0 .0 5 ;(0 .12± 0 .0 4vs 0 .18± 0 .0 6 ) g/cm2 ,P <0 .0 5〕 ;血清钙浓度显著升高〔(135 .9± 11.3vs 117.2± 6 .5 )mg/L ,P <0 .0 0 1〕 ,骨钙素水平显著升高〔(0 .0 7± 0 .0 4vs 0 .0 5± 0 .0 1) μg/L ,P <0 .0 5〕 ,维生素D3 水平显著降低〔(7.6± 1.9vs 11.6± 4 .1)μg/L ,P <0 .0 5〕 ,尿吡啶酚 /肌酐显著降低〔(4.8± 0 .8vs 75 .8± 6 0 .7)nmol/mmolCr,P <0 .0 1〕 ;而降钙素和PTH水平改变无统计学意义。相关性分析显示 ,血清NO与尿吡啶酚排泄呈负相关 (r= - 0 .74 ,
Objective To study the relationship between serum nitric oxide (NO) and early bone metabolism in diabetic rats. Methods Twenty SD rats were divided into two groups: one group was induced by streptozotocin (STZ DM) and the other was normal control group. The fasting blood glucose (FBG) HbA1c, serum insulin, systemic, femoral and lumbar spine BMD, markers of bone metabolism [serum calcium, osteocalcin, calcitonin, parathyroid hormone (PTH), vitamin D3 and urinary pyridinol / creatinine ratio] And serum NO levels. Results Compared with the normal control group, serum NO level was significantly increased in STZ DM rats 〔(51.3 ± 11.9 vs 38.1 ± 12.0) μmol / L, P <0.01〕; BMD in the whole body, femur and lumbar spine (0 .15 ± 0. 0 7 vs 0 .2 1 ± 0 .0 2) g / cm2, P <0. 01; (0 .16 ± 0 .0 2 vs 0 .19 ± 0 .0 3 ) g / cm2, P0.05; (0.12 ± 0.04 vs 0.18 ± 0.06) g / cm2, P <0.05); serum calcium concentration was significantly increased 〔(( 135.9 ± 11.3 vs 117.2 ± 6.5 (mg / L, P <0.01), and the level of osteocalcin was significantly increased 〔(0 .7 ± 0. 0 1) μg / L, P <0.05), the level of vitamin D3 was significantly lower (7.6 ± 1.9 vs 11.6 ± 4.1, p <0.05), the urine pyridinol / creatinine was significantly (4.8 ± 0.8 vs 75.8 ± 6.07) nmol / mmolCr, P <0.01). However, the changes of calcitonin and PTH were not statistically significant. Correlation analysis showed that serum NO was negatively correlated with urinary pyridinol excretion (r = - 0.74,