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目的：寻找胶质母细胞瘤１０号染色体长臂（１０ｑ）上可能存在肿瘤抑制基因的杂合性缺失（ＬＯＨ）区域，为发现和定位肿瘤抑制基因提供线索和依据。方法：应用聚合酶链反应（ＰＣＲ）方法分析１０例胶质母细胞瘤（ＧＢＭ）染色体１０ｑ上９个微卫星多态性标记的ＬＯＨ。结果：在９０％（９／１０例）肿瘤组织染色体１０ｑ上检出ＬＯＨ。以在１０ｑ～（２３）上的Ｄ１０ｓ２４９２－Ｄ１０ｓ５４１位点间和１０ｑ（２５－２６）上的Ｄ１０ｓ１７２３－Ｄ１０ｓ２１６位点间区域的ＬＯＨ频率最高。结论：染色体１０ｑ～（２３）上的Ｄ１０ｓ２４９２－Ｄ１０ｓ５４１位点间和１０～ｑ（２５－２６）上的Ｄ１０ｓｌ７２３－Ｄ１０ｓ２１６位点间区域可能存在与ＧＢＭ有关的多个肿瘤抑制基因。
OBJECTIVE: To find a possible heterozygous deletion (LOH) region of tumor suppressor gene on the long arm of glioblastoma 10q (10q), and to provide clues and evidences for the discovery and localization of tumor suppressor genes. Methods: Polymerase chain reaction (PCR) was used to analyze 9 microsatellite polymorphism markers LOH on chromosome 10q of 10 glioblastoma (GBM). Results: LOH was detected on chromosome 10q of 90% (9/10) tumor tissues. The frequency of LOH in the region between D10s2492-D10s541 on 10q ~ (23) and D10s1723-D10s216 on 10q (25-26) was the highest. CONCLUSION: There may exist multiple tumor suppressor genes related to GBM in the region between D10s2492-D10s541 on chromosome 10q ~ (23) and D10s1723-D10s216 on 10 ~ q (25-26).