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近来,高效液相色谱法作为分离分析的重要手段,已得到广泛应用,其中反相色谱分离技术的应用尤显突出。此法所用移动相配制简单,采用不同混合比的甲醇和水进行淋洗,能使样品有很好的分离效果。甲醇和水,价廉且容易得到,与其他有机溶剂相比,毒性较低,还具有优良的透紫外光性能,所以用作色谱移动相颇为理想。按理,甲醇的最大吸收峰在183毫微米,其克分子吸光系数也只有150,因此205毫微米处以上可视作透明区。然而,市售试剂内常含有微量紫外强吸收
Recently, as an important means of separation and analysis, high performance liquid chromatography has been widely used, of which the application of reverse phase chromatographic separation technology is particularly prominent. The mobile phase used in this method is simple to prepare, with different mixing ratios of methanol and water for rinsing, which can make the sample have a good separation effect. Methanol and water, inexpensive and readily available, have lower toxicity than other organic solvents and have excellent UV-transmission properties, making them ideal for use as a mobile phase for chromatography. Logically, the maximum absorption peak of methanol at 183 nm, the molecular absorption coefficient is only 150, so above 205 nm can be seen as a transparent area. However, commercial reagents often contain traces of UV-light absorption