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呼吸窘迫综合症(RDS)是临床上常见的一种疾病,发病机理复杂。为探讨其发病机理,我们用10只日本大耳兔复制出海水型RDS动物模型,另取7只作对照,取肾皮质在光镜和电镜下进行胞嘧啶单核苷酸酶(CMP酶)和细胞色素氧化酶研究,并对酶活性反应作相对定量测定。恒冷箱切片时两组动物肾皮质同时裱在同一玻片上,同一条件下进行酶反应,用我们自行研制的真彩色医学图像分析系统进行酶活性反应的平均灰度测量(灰度分0-256级,以平均灰度代表酶反应强度,灰度值越大,表示酶反应强度越低)。以
Respiratory distress syndrome (RDS) is a common clinical disease, the pathogenesis of complex. To explore its pathogenesis, we use 10 Japanese large-eared rabbits to replicate the seawater-type RDS animal model, and take 7 as a control. Take the renal cortex under light and electron microscopy for cytosine mononucleotidase (CMP) And cytochrome oxidase research, and the relative quantitative determination of enzyme activity. When the cryostat was sliced, the renal cortices of both groups of animals were mounted on the same slide at the same time, and enzyme reaction was carried out under the same conditions. The average gray level of the enzyme activity reaction was measured by our own true color medical image analysis system (grayscale 0- 256 levels, with the average gray level on behalf of the enzyme reaction intensity, the greater the gray value, the lower the enzyme reaction intensity). To