论文部分内容阅读
采用分步法SDS-PAGE和改良A-PAGE分析了49份小麦农家品种等资源的Glu-1,Glu-3,Gli-1位点基因组成.结果表明,Glu-1位点编码的HMW-GS组成共检测到14种图谱,其中占比例最大图谱(N,7+8,2+12)达44.9%,其次是(N,7+9,2+12)和(1,7+8,2+12)各占14.3%,(1,7+9,5+10)和(2*,7+9,2+12)各占4.1%,其余9种图谱各占2.0%;Glu-3位点编码的LMW-GS组成共检测到25种图谱,(a,j,c)占12.2%,(a,g,c)占10.2%,(new,g,c)占8.2%,(a,g,a),(a,j,a),(f,g,c)和(d,j,a)各占6.1%,(f,j,a),(d,g,c),(a,f,c)和(f,f,c)各占4.1%,其余14种图谱各占2.0%,Glu-A3位点新发现的基因图谱占14.3%;Gli-1位点编码的醇溶蛋白组成共检测到40种图谱,(a,f,l)和(f,l,i)各占6.1%,(f,l,a),(new,c,i),(b,g,l)和(l,l,a)各占4.1%,其它34种图谱各占2.0%,Gli-1位点新发现的基因图谱占16.3%;扬白麦、迟亚草、葫芦头麦、红秃头、红粒白芒、红火麦、矮孟牛IV中在Glu-3或Gli-1位点含有新发现的等位基因.
Glu-1, Glu-3 and Gli-1 loci in 49 wheat cultivars and other resources were analyzed by step-by-step SDS-PAGE and modified A-PAGE.The results showed that the HMW- A total of 14 patterns were detected in the GS composition, with the largest proportion (N, 7 + 8, 2 + 12) reaching 44.9%, followed by (N, 7 + 9,2 + 12) and (1,7 + 8, 2 + 12) accounted for 14.3%, (1,7 + 9,5 + 10) and (2 *, 7 + 9,2 + 12) each accounted for 4.1%, the remaining 9 kinds of maps each accounted for 2.0%; Glu-3 (A, j, c) accounted for 10.2%, (a, g, c) accounted for 10.2%, (new, g, c) accounted for 8.2% (f, j, a), (d, g, c) and (d, j, a) represent 6.1% (a, f, c) and (f, f, c) each accounted for 4.1%, and the other 14 maps accounted for 2.0% each. The newly discovered gene map at Glu-A3 accounted for 14.3% (A, f, l) and (f, l, i) each accounted for 6.1%, (f, l, a), (new, c, i) g, l) and (l, l, a) accounted for 4.1%, the other 34 kinds of maps each accounted for 2.0%, Gli-1 locus accounted for 16.3% of the newly discovered gene map; Young white wheat, Late Asian grass, cucurbit The newly found alleles were found at the Glu-3 or Gli-1 loci in wheat, red bald, red white white mandarin, red fire malted, and dwarf Mongo IV.