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在获得带有结构基因的质粒ｐＰＣ４１和穿梭质粒ｐＳＡ３的基础上，制备目的基因并与载体ＤＮＡ进行体外连接，使重组体导入乳链球菌ＬＭ０２３０受体细胞，为研制防龋疫苗获得携带变链菌表面抗原基因的重组乳链球菌。
After obtaining the plasmid pPC41 with the structural gene and the shuttle plasmid pSA3, the target gene was prepared and ligated with the vector DNA in vitro, and the recombinant was introduced into the recipient cell of LM0230 of Streptococcus agalactiae to obtain Streptococcus mutans Surface antigen genes of recombinant Streptococcus agalactiae.