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通过氯化铈 (Ce Cl3)沉淀的电镜细胞化学法 ,对比观察了抗寒冬小麦幼苗和冷敏感春小麦幼苗质膜 Ca2 + -ATPase活性的变化 ,结果显示 :2 0℃下生长的冬小麦幼苗 ,Ca2 + - ATPase活性主要定位于质膜上。2℃下 ,随处理时间的延长 (3h、12 h) ,质膜 Ca2 + - ATPase活性反应逐步增强。2℃下处理 3d,质膜上仍存在酶反应产物 ,质膜 Ca2 + - ATPase活性高于对照。2 0℃下生长的春小麦幼苗 ,其质膜 Ca2 + - ATPase活性水平高于同样条件处理下的冬小麦幼苗的酶活性水平。2℃处理 3h,春小麦幼苗质膜上酶活性反应明显增强。2℃处理 12 h,酶活性反应迅速降低。2℃下处理 3d,春小麦幼苗质膜上酶反应产物明显少于对照 ,质膜 Ca2 + - ATPase已接近失活。结果表明 ,低温逆境下质膜 Ca2 + - ATPase活性的大小及其稳定性 ,是决定植株本身抗寒能力大小的关键
The changes of plasma membrane Ca2 + -ATPase activity in cold-sensitive and spring wheat-resistant winter wheat seedlings were compared by electron microscopy with CeCl3 precipitation. The results showed that the Ca2 + + - ATPase activity mainly located on the plasma membrane. At 2 ℃, the plasma membrane Ca2 + - ATPase activity gradually increased with the prolongation of treatment time (3 h, 12 h). At 2 ℃ for 3 days, the enzymatic reaction still existed on the plasma membrane, and the plasma membrane Ca2 + - ATPase activity was higher than that of the control. The plasma membrane Ca2 + - ATPase activity of spring wheat seedlings grown at 20 ℃ was higher than that of winter wheat seedlings under the same conditions. 2 ℃ treatment 3h, spring wheat seedlings membrane plasma membrane enzyme reaction was significantly enhanced. 2 ℃ for 12 h, enzyme activity decreased rapidly. At 2 ℃ for 3 days, the products of enzymatic reaction on the plasma membrane of spring wheat seedlings were obviously less than those of the control, and the plasma membrane Ca2 + - ATPase was almost inactivated. The results showed that the size and stability of Ca2 + - ATPase in plasma membrane under low temperature stress were the key factors to determine the ability of plants to resist cold