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目的通过NMR技术探讨丹红注射液对自发性高血压大鼠血清代谢物的影响。方法将8周龄的SPF级SHR大鼠实验大鼠随机分为两组:SHR组(自发高血压大鼠组)、DH组(丹红注射液对SHR大鼠干预组),另选同龄Wister-Kyoto大鼠(WEY组)作为血压正常对照组,每组均为6只。治疗组给予丹红注射液,SHR组给予同等剂量无菌生理盐水,均按10m L/kg体重大鼠腹腔注射给药,连续给药28d。检测各组大鼠血压、心率等生理指标,并采用NMR测定各组大鼠血清中代谢物水平。结果与WKY组比较,SHR组合DH组大鼠心率、血压均有不同程度升高,结果有统计学意义;DH组与SHR组比较,收缩压和舒张压均有降低的趋势,但差异无统计学意义;与WKY大鼠比较,SHR大鼠血清中有15种代谢物水平发生了变化,其中8种降低,7种升高;与WKY大鼠比较,SHR大鼠血清中有15种代谢物水平发生了变化,降低有8种,升高有7种。结论丹红注射液不能有效降低SHR大鼠血压,但能逆转大鼠血清中多种代谢物水平,改善体内环境,对机体产生保护效应。
Objective To investigate the effect of Danhong Injection on serum metabolites in spontaneously hypertensive rats by NMR. Methods Eight-week-old SPF SHR rats were randomly divided into two groups: SHR group (spontaneously hypertensive rats), DH group (Danhong injection SHR rats intervention group), the same age Wister - Kyoto rats (WEY group) as a normal blood pressure control group, each group of 6. The treatment group were given Danhong injection, SHR group were given the same dose of sterile saline, 10m L / kg weight rats were intraperitoneally administered for continuous administration of 28d. The blood pressure, heart rate and other physiological indexes of rats in each group were detected. The levels of metabolites in serum of rats in each group were determined by NMR. Results Compared with WKY group, the heart rate and blood pressure in SHR combined DH group increased with different degrees, the results were statistically significant; systolic and diastolic blood pressure tended to decrease in DH group compared with SHR group, but the difference was not statistically significant Significance of the changes; Compared with WKY rats, SHR rat serum levels of 15 metabolites have changed, of which 8 were reduced, 7 were increased; compared with WKY rats, SHR rat serum 15 metabolites The levels have changed, there are 8 kinds of lower, 7 kinds of higher. Conclusion Danhong injection can not effectively reduce the blood pressure of SHR rats, but it can reverse the levels of many metabolites in the serum of rats and improve the environment in vivo and have a protective effect on the body.