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目的 :对比分析多巴胺 1受体激动剂非诺多泮对兔肺动脉和肠系膜动脉的影响。方法 :采用cAMP含量放射免疫测定法测定DA1受体激动剂非诺多泮对肾动脉和肠系膜动脉cAMP的影响。结果 :非诺多泮可剂量依赖性增加肠、肺动脉cAMP的生成量 ,然而 ,肠动脉cAMP的生成量显著高于肺动脉cAMP的生成量。选择性多巴胺(DA1)受体阻断剂SCH2 3 3 90能够阻断非诺多泮所引起的肺动脉和肠动脉cAMP生成量增加 ,多巴胺 2 (DA2 )受体阻断剂Domperidone则不影响非诺多泮的作用。结论 :在兔肺和肠动脉均存在有刺激AC活性的DA1受体。但肺动脉DA1受体的位点数明显少于肠动脉DA1受体位点数 ,从而提示肺动脉DA1受体的生理作用弱于肠动脉。
OBJECTIVE: To compare the effects of fenotrodamine, a dopamine 1 receptor agonist, on pulmonary and mesenteric arteries in rabbits. Methods: cAMP content radioimmunoassay was used to determine the effect of DA1 receptor agonist fenoldopan on cAMP in renal arteries and mesenteric arteries. Results: Fenoldopam dose-dependently increased the production of cAMP in the intestine and pulmonary artery. However, the production of cAMP in the intestinal artery was significantly higher than that of the cAMP in the pulmonary artery. SCH2 3 3 90, a selective dopamine (DA1) receptor blocker, blocked the increase in cAMP production in both pulmonary and enteric arteries induced by fenoldopam, while Domperidone, a dopamine 2 (DA2) receptor blocker, The role of multi-pan CONCLUSION: There are DA1 receptors that stimulate AC activity in rabbit lung and intestine. However, the number of DA1 receptors in the pulmonary artery was significantly less than that of the DA1 receptor in the intestinal artery, suggesting that the physiological role of the DA1 receptor in the pulmonary artery is weaker than that of the intestinal artery.