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目的 探讨谷胱甘肽S 转移酶M1(GSTM1)基因型与接触石棉的工人血液发生脂质过氧化的关系。方法 选择 94名石棉作业工人及 5 1名对照工人作为研究对象 ,通过问卷调查收集每个研究对象的一般情况、职业史等。同时测定血浆丙二醛 (MDA)的含量 ,分析淋巴细胞DNA中GSTM1的基因型。结果 石棉作业工人血浆MDA含量为 (0 .2 83± 0 .0 5 4)nmol/L ,明显高于对照组工人 [(0 .16 3± 0 .0 5 3)nmol/L],差异有显著性 (P <0 .0 1) ,但MDA含量与工龄和累积石棉接触剂量之间的相关关系不明显 ;对照组工人携带GSTM1- /-者血浆中的MDA含量 [(0 .190± 0 .0 34 )nmol/L]明显高于携带GSTM1+/+者[(0 .138± 0 .0 5 5 )nmo/L],差异有显著性 (P <0 .0 1) ,而石棉作业工人组虽也有类似趋势 ,但无统计学差异 (P >0 .0 5 ) ;对于石棉作业工人来说 ,在工龄相同或累积石棉接触剂量相同时 ,携带GSTM1- /-者的血浆MDA含量均高于携带GSTM1+/+者 ,但差异均无显著性 (P >0 .0 5 )。结论 接触石棉和GSTM1- /-基因型均与作业工人机体内脂质过氧化有关 ,但石棉的作用可能大于GSTM1基因型的作用。
Objective To investigate the relationship between glutathione S transferase M1 (GSTM1) genotype and blood lipid peroxidation in workers exposed to asbestos. Methods 94 asbestos workers and 51 control workers were selected as research objects. The general situation and occupational history of each research object were collected through questionnaire survey. Plasma malondialdehyde (MDA) levels were also determined, and genotypes of GSTM1 in lymphocyte DNA were analyzed. Results The level of plasma MDA in asbestos-exposed workers was (0.23 ± 0. 054) nmol / L, which was significantly higher than that in control group [(0.163 ± 0.053) nmol / L] (P <0.01), but there was no significant correlation between the content of MDA and the length of service life and cumulative exposure to asbestos. The content of MDA in plasma of workers carrying GSTM1- / [ .0 34) nmol / L] was significantly higher than those carrying GSTM1 + / + [(0.138 ± 0.055) nmo / L], with significant difference (P <0.01) There was no significant difference between the two groups (P> 0.05). For asbestos workers, the plasma levels of MDA in GSTM1- / GSTM1- / GSTM1- / In carrying GSTM1 + / +, but the difference was not significant (P> 0.05). Conclusions Exposure to asbestos and GSTM1- / - genotypes is associated with lipid peroxidation in workers’ bodies, but the role of asbestos may be greater than that of GSTM1 genotypes.