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目的：用微板核酸杂交－ＥＬＩＳＡ技术检测皖北地区不同人群ＴＴＶ感染情况。方法：用ＰＣＲ法将 患者血清标本中的 ＴＴＶ ＤＮＡ扩增后，与两条特异性探针夹心杂交，通过酶联显色检测血清标本中的ＴＴＶ ＤＮＡ，进行分型并与套式ＰＣＲ方法比较。结果：ＴＴＶ在学生、血透患者、肝病患者中的阳性率分别为３．３％， １６．７％和１３．５％，前者与后两者比较差异均有显著意义（Ｐ＜０．０５）；１４例非甲－非戊（ＮＡ－ＮＥ）肝炎的ＴＴＶ阳性 率为１４．３％。本地区流行的ＴＴＶ可分为两个主要的基因型，即Ｉ型（６６．７％）、Ⅱ型（２５％），并存在混合感染 （８．３％）。微板核酸杂交－ＥＬＩＳＡ与套式ＰＣＲ方法的相符率为９８．ｌ％（２０５／２０９）． 结论：本地区一般人群、血透 和肝病患者中存在ＴＴＶ感染，后两者为感染的高危人群，ＴＴＶ的基因型以Ｉ型为主。ＴＴＶ不是本地 ＮＡ－ＮＥ肝 炎的主要病因。微板核酸杂交－ＥＬＩＳＡ技术具有灵敏性高、操作简单、易自动化的特点。
OBJECTIVE: To detect TTV infection in different populations in northern Anhui by ELISA-microplate nucleic acid-ELISA. Methods: The TTV DNA in serum samples of patients was amplified by PCR and then hybridized with two specific probes. The TTV DNA in serum samples was detected by enzyme-linked immunosorbent assay and compared with the nested PCR method . Results: The positive rates of TTV in students, hemodialysis patients and liver disease patients were 3.3%, 16.7% and 13.5%, respectively. There was significant difference between the former and the latter (P <0.05 ). The positive rate of TTV in 14 non-A-NA patients was 14.3%. The prevalence of TTV in the region can be divided into two major genotypes, namely type I (66.7%), type II (25%), and the presence of mixed infection (8.3%). The coincidence rate of microplate nucleic acid-ELISA with nested PCR method was 98. l% (205/209). CONCLUSION: TTV infection exists in the general population, hemodialysis patients and liver disease patients in the region. The latter two are at high risk of infection. The genotype of TTV is mainly type I. TTV is not the leading cause of native NA-NE hepatitis. Microplate nucleic acid hybridization-ELISA technology has the characteristics of high sensitivity, simple operation and easy automation.