用流式细胞光度术(FcM)测定了冬凌草甲素和博莱霉素A_5联合应用对艾氏腹水癌细胞增殖周期的影响。发现冬凌草甲素可阻滞细胞M→G_1或M→G_1和G_2→M的进程,导致G_2→M%的增加,博莱霉素A_2阻断G_2→M的进行,使G_2%增加,合用后G_2+M%的构成比随时延长而大于单用组,这些现象有利于博莱霉素A_5等对M,G_2敏感时相的细胞杀伤。 The effect of oridonin and bleomycin A-5 on the proliferation cycle of Ehrlich ascites carcinoma cells was determined by flow cytometry (FcM). It was found that Oridonin can block the progression of cells M→G_1 or M→G_1 and G_2→M, resulting in an increase in G_2→M%, while Bleomycin A_2 blocks G_2→M and increases G_2%. After combined use, the composition ratio of G_2+M% was prolonged at any time and was greater than that of the single-use group. These phenomena are beneficial to the cell killing of M, G_2 sensitive phases such as bleomycin A_5.