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目的 筛选并克隆在哈萨克族食管鳞癌与正常食管组织之间差异表达的基因。方法 (1)应用抑制性消减杂交(SSH)进行基因差异表达分析,构建哈族食管鳞癌组织和正常食管黏膜组织之间差异表达的cDNA消减文库;(2)克隆、鉴定食管癌组织特异表达的基因;(3)登录Genbank,运用 Blastn程序进行同源性分析。结果 成功构建了消减效率高的哈族食管鳞癌 cDNA消减文库,对其中 6 个克隆的插入cDNA片断进行测序,经检索Genbank表明:这些差异表达基因与跨膜受体、乳腺癌转录因子、蛋白剪接基因及染色体1、8不同区域有较高的同源性。结论 通过抑制性消减杂交技术构建了哈族食管鳞癌cDNA消减文库,并分析鉴定了部分差异表达基因,为进一步筛选鉴定食管鳞癌特异性基因及其全长克隆、功能研究等提供了依据。
Objective To screen and clone differentially expressed genes in Kazakh esophageal squamous cell carcinoma and normal esophageal tissues. Methods (1) Differential subtractive hybridization (SSH) was used to detect the expression of differentially expressed genes in Esophageal squamous cell carcinoma and normal esophageal squamous cell carcinoma tissues. (2) Cloning and identification of esophageal cancer tissue-specific expression (3) Log on to Genbank and perform homology analysis using Blastn program. Results The cDNA subtraction library of Ha ethnic esophageal squamous cell carcinoma with high abatement efficiency was successfully constructed. The inserted cDNA fragments of 6 clones were sequenced. Genbank showed that these differentially expressed genes were associated with transmembrane receptors, breast cancer transcription factors, Splicing genes and chromosomes 1,8 different regions have a higher homology. Conclusion Suppression subtractive hybridization (SSH) technique was used to construct cDNA subtractive library of Ha ethnic esophageal squamous cell carcinoma and to analyze and identify some differentially expressed genes. This study may provide a basis for further screening and identification of esophageal squamous cell carcinoma specific genes and their full length clones.