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用60Coγ射线辐照粑型温敏核不育系2177S,获得了一批常见的叶绿素突变体。M2代按苗计,叶绿素总突变频率为0.262%,其中只有4.50%可以存活并遗传,从中筛选出33个综合性状优良的籼型温敏核不育叶绿素突变系。经鉴定,这些叶绿素突变受基因控制,比较稳定,有的只在苗期表达并逐渐转为正常,有的则在全生育期表达。与2177S相比,农艺性状都有不同程度变化,但温敏不育特性基本不受影响。遗传分析表明,所有的突变体与亲本正反交时,杂种F1均为绿色植株,杂种F2群体中,正常绿色株与对应叶绿素突变株分离比值符合3:1。证明这类突变是由1对隐性细胞核基因所控制。
A series of common chlorophyll mutants were obtained by irradiating Irradiance-type thermo-sensitive genic male sterile line 2177S with 60Coγ ray. According to the seedling number in M2 generation, the total chlorophyll mutation frequency was 0.262%, of which only 4.50% could survive and inherit, and 33 indica thermo-sensitive genic chlorophyll mutation lines with good comprehensive characters were screened out. It has been identified that these chlorophyll mutations are genetically controlled and relatively stable, while others are only expressed at seedling stage and gradually turned to normal, while others are expressed during the whole growth period. Compared with 2177S, agronomic traits varied to some extent, but the thermo-sensitive sterile characters were basically unaffected. Genetic analysis showed that all the F1 hybrids were green plants in reciprocal cross between parents and parents, and the ratio of normal green plants to corresponding chlorophyll mutants was 3: 1 in F2 hybrids. Proved that these mutations are controlled by a pair of recessive nuclear genes.