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目的:探讨白藜芦醇对心肌缺血再灌注损伤大鼠炎症和氧化应激的影响及可能的作用机制。方法:30只SD大鼠随机分为假手术组(Sham组)、缺血再灌注组(MI/R组)、白藜芦醇预处理组(MI/R+Res组),采用结扎左冠状动脉前降支起始部制作大鼠心肌缺血再灌注损伤模型,Sham组穿针后不结扎,MI/R组于缺血前15min以及再灌注前1 min舌下静脉注入10 mg/kg生理盐水,MI/R+Res组静脉注入等量白藜芦醇,对比三组心功能指标左心室舒张末期压(LVEDP)、左心室射血分数(LVEF)、左室内压力变化最大上升和下降速率(±dp/dt max),心肌梗死面积,心肌损伤标志物肌酸激酶(CK)乳酸脱氢酶(LDH),冠状动脉炎症标志物髓过氧化酶(MPO),氧化应激指标超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA),Western blot检测Nrf2/ARE信号通路蛋白Nrf2和HO-1蛋白表达。结果:与Sham组比较,MI/R组LVEDP、心肌梗死面积、CK、LDH、MPO、MDA显著升高,而LVEF、+dp/dt max、-dp/dt max、SOD、GSH-PX显著降低,差异有统计学意义(P<0.05);MI/R+Res组LVEDP、心肌梗死面积、CK、LDH、MPO、MDA均显著低于MI/R组,LVEF、+dp/dt max、-dp/dt max、SOD、GSH-PX均显著高于MI/R组,差异有统计学意义(P<0.05);Western blot显示Sham组Nrf2和HO-1蛋白表达较少,MI/R组Nrf2和HO-1蛋白表达显著增加,与MI/R组比较,MI/R+Res组Nrf2和HO-1蛋白表达显著增加,差异有统计学意义(P<0.05)。结论:白藜芦醇可能通过激活Nrf2/ARE信号通路降低心肌缺血再灌注损伤大鼠炎症和氧化应激。
Objective: To investigate the effect of resveratrol on inflammation and oxidative stress in myocardial ischemia-reperfusion injury rats and its possible mechanism. Methods: Thirty SD rats were randomly divided into Sham group, MI group, and resveratrol preconditioning group (MI / R + Res group) The model of myocardial ischemia-reperfusion injury was made at the beginning of the anterior descending artery in rats. Sham group was not ligated after injection of the needle. The MI / R group was injected with 10 mg / kg sublingual vein 15 min before ischemia and 1 min before reperfusion Saline and MI / R + Res group were given intravenous injection of the same amount of resveratrol. The left ventricular end-diastolic pressure (LVEDP), left ventricular ejection fraction (LVEF), maximum increase and decrease of left ventricular pressure (± dp / dt max), area of myocardial infarction, myocardial injury markers creatine kinase (CK) lactate dehydrogenase (LDH), markers of coronary artery myeloperoxidase (MPO), oxidative stress indicators superoxide The expressions of Nrf2 and HO-1 proteins in Nrf2 / ARE signaling pathway were detected by Western blot. The levels of SOD, GSH-Px and MDA were determined by Western blot. Results: LVEDP, myocardial infarct size, CK, LDH, MPO and MDA in MI / R group were significantly higher than those in sham group, while LVEF, + dp / dt max, -dp / dt max, SOD and GSH-PX were significantly decreased (P <0.05). LVEDP, myocardial infarct size, CK, LDH, MPO and MDA in MI / R + Res group were significantly lower than those in MI / R group, LVEF, + dp / dt max, -dp / dt max, SOD and GSH-PX in MI / R group were significantly higher than those in MI / R group (P <0.05). Western blot showed that Nrf2 and HO- Compared with MI / R group, the expression of HO-1 protein in MI / R + Res group increased significantly, the difference was statistically significant (P <0.05). Conclusion: Resveratrol may reduce inflammation and oxidative stress in rats with myocardial ischemia-reperfusion injury by activating Nrf2 / ARE signaling pathway.