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将１０％福尔马林１５０～２００μｌ注入大鼠一侧后肢足底，用注入等量０．９％生理盐水作为对照，分别于刺激后５、１５、３０、６０、９０、１２０ｍｉｎ和１２、２４、４８ｈ取材，进行免疫组化反应（ＡＢＣ法），观察脊髓腰骶段后角浅层及后连合核区的ＳＰ、ＣＧＲＰ、Ｌ－ＥＮＫ样纤维和终末的分布，并随机抽样进行图像分析．结果显示：在福尔马林刺激后，脊髓后角浅层和后连合核区的ＳＰ、ＣＧＲＰ样阳性纤维和终末在５～６０ｍｉｎ明显减少，而到１．５～１２ｈ却呈增加趋势，１２ｈ后逐渐恢复正常．而Ｌ－ＥＮＫ样纤维和终末始终无明显变化．本文结合文献讨论了上述变化与痛与镇痛机制的关系。
150 ~ 200μl 10% formalin was injected into the hindlimb of rat hindlimb. The rats were injected 0.9% normal saline as control. After 5, 15, 30, 60, 90, 120min and 12, 24,48h, immunohistochemical reaction (ABC method) was used to observe the distribution of SP, CGRP, L-ENK-like fibers and terminals in the superficial and posterior commissural nucleus of the lumbosacral segment, and random sampling Image analysis. The results showed that SP and CGRP-like positive fibers and terminals in superficial and posterior commissural nucleus of spinal cord decreased significantly after 5 ~ 60min, but increased from 1.5 ~ 12h after formalin stimulation , Gradually returned to normal after 12 hours. The L-ENK-like fiber and the terminal has no significant change. This article discusses the relationship between the above changes and pain and analgesic mechanisms in combination with the literature.