论文部分内容阅读
为观察表皮生长因子 ( epidermalgrowth factor,EGF)对成年水牛晶状体上皮细胞增殖作用的影响。将不同浓度 EGF作用于体外培养的成年水牛晶状体上皮细胞 ,采用 MTT法测定细胞的增殖能力。结果 :EGF在浓度为 1ng/ m L和 1 0 ng/ m L作用的前 3 d促增殖作用逐渐加强 ,呈现时间依赖性 ,且在第 3 d达到最大促增殖效果。不同浓度的 EGF对晶状体上皮细胞的增殖作用不同 ,浓度在 1 0 ng/ m L作用 2 4h后即有明显的促增殖作用 ( P <0 .0 5) ,而作用72 h后最低的有效浓度为 1 ng/ m L,而且 2 50ng/ m L EGF有最大促增殖作用。结论 :EGF是诱导成年水牛晶状体上皮细胞增殖的重要因素。
To observe the effects of epidermal growth factor (EGF) on the proliferation of adult buffalo lens epithelial cells. Effects of different concentrations of EGF on cultured buffalo lens epithelial cells in vitro were measured by MTT assay. RESULTS: EGF promoted the proliferation in the first 3 days at a concentration of 1ng / m L and 10 ng / m L, and showed a time-dependent manner and achieved the maximum proliferative effect on the 3rd day. Different concentrations of EGF had different proliferative effects on lens epithelial cells. The concentration of EGF enhanced the proliferation of lens epithelial cells at a concentration of 10 ng / mL for 24 h (P <0.05), while the lowest effective concentration after 72 h 1 ng / m L, and 2 50 ng / m L EGF has the maximum proliferation. Conclusion: EGF is an important factor inducing the proliferation of adult buffalo lens epithelial cells.