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目的探讨幽门螺杆菌(Helicobacterpylori,H.pylon)感染与胃癌、癌旁及胃炎黏膜中hMSH2,hMLH1和p53基因表达的关系,进一步了解H.pylori在胃癌发生中的作用。方法采用快速尿素酶方法检测H.pylori;采用免疫组化SP法检测hMSH2,hMLH1和p53基因表达。结果(1)hMSH2在胃癌中的表达阳性率(62.7%)显著高于癌旁(29.4%),胃炎黏膜(32.4%)和正常对照(30.0%)(P<0.001)。其中在低分化腺癌中阳性率(76.4%)显著高于高中分化腺癌(54.3%)和黏液癌(53.1%)(P<0.05);hMLH1在胃癌中的表达阳性率(64.3%)显著低于癌旁(84.4%),胃炎黏膜(82.4%)和正常对照(80.0%)(P<0.01)。其中在黏液癌的阳性率(43.7%)显著低于其他两种腺癌(78.2%,64.7%)(P<0.01);p53在胃癌中的表达阳性率(51.9%)显著高于癌旁(3.1%),胃炎黏膜(0.0%)和正常对照(0.0%)(P<0.001)。其中,在高中分化腺癌的阳性率(32.6%)显著低于黏液癌(68.7%)和低分化腺癌(58.8%)(P<0.05)。(2)在全部被检组织中,H.pylori感染组hMSH2和hMLH1的表达阳性率均低于相应的非感染组。其中,胃癌H.pylori感染组hMSH2的表达阳性率(52.8%)显著低于非感染组(74.5%)(P<0.05)。胃癌H.pylori感染组p53的表达阳性率(61.4%)显著高于非感染组(40.6%)(P<0.05)。结论胃癌的发生发展可能与hMSH2,hMLH1和p53基因的异常表达有关;H.pylori感染影响这三种基因的正常表达,这可能是H.pylori致癌的机制之一。
Objective To investigate the relationship between Helicobacter pylori (H.pylon) infection and the expression of hMSH2, hMLH1 and p53 in gastric mucosa, paracancer and gastritis mucosa and to further understand the role of H.pylori in gastric carcinogenesis. Methods Rapid urease was used to detect H.pylori. The expression of hMSH2, hMLH1 and p53 was detected by immunohistochemical SP method. Results (1) The positive rate of hMSH2 expression in gastric cancer (62.7%) was significantly higher than that in adjacent cancer (29.4%), gastritis mucosa (32.4%) and normal control (30.0%) (P <0.001). The positive rate of hMLH1 in poorly differentiated adenocarcinoma (76.4%) was significantly higher than that in high-differentiated adenocarcinoma (54.3%) and mucinous carcinoma (53.1%) (P <0.05) (84.4%), gastritis mucosa (82.4%) and normal control (80.0%) (P <0.01). (43.7%) was significantly lower than that of the other two kinds of adenocarcinoma (78.2%, 64.7%) (P <0.01). The positive expression rate of p53 in gastric carcinoma (51.9%) was significantly higher than that in adjacent tissues 3.1%), gastritis mucosa (0.0%) and normal control (0.0%) (P <0.001). Among them, the positive rate of differentiated adenocarcinoma in high school (32.6%) was significantly lower than that in mucinous carcinoma (68.7%) and poorly differentiated adenocarcinoma (58.8%) (P <0.05). (2) The positive rates of hMSH2 and hMLH1 in H.pylori infection group were lower than those in non-infected group in all the tested tissues. Among them, the positive rate of hMSH2 expression in H.pylori-infected gastric cancer group (52.8%) was significantly lower than that in non-infected group (74.5%) (P <0.05). The positive rate of p53 expression in H.pylori infection group (61.4%) was significantly higher than that in non-infected group (40.6%) (P <0.05). Conclusions The occurrence and development of gastric cancer may be related to the abnormal expression of hMSH2, hMLH1 and p53 genes. H.pylori infection affects the normal expression of these three genes, which may be one of the mechanisms of carcinogenesis of H.pylori.