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目的 观察新生大鼠脑发育成熟过程中糖皮质激素代谢酶 11β 羟基类固醇脱氢酶Ⅰ型 (11β HSD1)的变化。 方法 免疫组织化学和Western印迹杂交的方法。 结果 免疫组织化学染色表明 ,11β HSD1分布于新生大鼠大脑皮层的各个层次 ,但以Ⅱ层 (外颗粒层 )含量为最高、Ⅲ (锥体细胞层 )、Ⅳ (内颗粒层 )层 11β HSD1的含量次之。海马的各个区域和齿状回也均有 11β HSD1的分布。Western印迹杂交分析表明 ,新生鼠顶叶皮层、海马和下丘脑 11β HSD1的表达在出生后 2周内表达一直比较高 ,第 15d时其表达开始下降。 结论 提示新生鼠大脑 11β HSD1的表达可能与糖皮质激素促进脑组织的发育和成熟有关。
Objective To observe the changes of glucocorticoid metabolic enzyme 11β hydroxysteroid dehydrogenase type 1 (11βHSD1) during brain development and maturation in neonatal rats. Methods Immunohistochemistry and Western blot hybridization methods. Results Immunohistochemical staining showed that 11β HSD1 was distributed in all layers of cerebral cortex of neonatal rats. However, the content of 11β HSD1 in layer Ⅲ (pyramidal cell layer), layer Ⅳ (inner granular layer) The content of the second. The distribution of 11β HSD1 was also found in all regions of the hippocampus and dentate gyrus. Western blot analysis showed that expression of 11β HSD1 in the parietal cortex, hippocampus and hypothalamus of neonatal rats was consistently high within 2 weeks after birth, and began to decline on the 15th day. Conclusions The expression of 11β HSD1 in neonatal rat brain may be related to the promotion of brain development and maturation by glucocorticoid.