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【目的】探讨桃叶鸦葱总黄酮对脑缺血再灌注小鼠脑组织损伤的修复作用及其机制。【方法】采用结扎右侧颈总动脉制备脑缺血再灌注小鼠模型。将96只清洁级雄性健康昆明种小鼠随机分成假手术组,模型组,桃叶鸦葱总黄酮高、中、低剂量组(剂量分别为400、200、100 mg·kg-1·d-1),依达拉奉组(剂量为6 mg·kg-1·d-1),每组16只。再灌注后72 h,各组小鼠进行神经功能损伤评分;实验结束后,采用氯化-2,3,5-三氯苯基四氮唑(TTC)染色法测定各组小鼠脑梗死范围,采用硫代巴比妥酸(TBA)法测定脑组织内丙二醛(MDA)含量,采用羟胺法测定超氧化物歧化酶(SOD)活性,采用5,5-二硫二硝基苯甲酸(DTNB)比色法检测谷胱甘肽过氧化物酶(GSH-Px)活性。【结果】与假手术组比较,模型组小鼠神经功能损伤评分明显增加,病理表现为脑梗死,脑组织内MDA含量明显升高,SOD、GSH-Px活性明显降低,差异均有统计学意义(P<0.01)。与模型组比较,桃叶鸦葱总黄酮高、中、低剂量组小鼠神经功能损伤评分明显减少,脑梗死面积明显缩小,脑组织内MDA含量明显降低,SOD、GSH-Px活性显著升高,均表现出明显的量效依赖关系;其中,桃叶鸦葱总黄酮高、中剂量组效果更明显(均P<0.05),且与依达拉奉组比较,差异无统计学意义(P>0.05)。【结论】桃叶鸦葱总黄酮对小鼠脑缺血再灌注损伤具有修复作用,可能与其具有抗氧化活性的功能有关。
【Objective】 To investigate the effect and mechanism of total flavonoids from Pterocarya variegata on cerebral injury induced by cerebral ischemia-reperfusion in mice. 【Methods】 A mouse model of cerebral ischemia-reperfusion was established by ligation of the right common carotid artery. Ninety-six clean male Kunming mice were randomly divided into sham-operated group, model group, total flavonoids of high-, middle-, and low-dose flavonoids (400, 200, 100 mg·kg-1·d- 1) In the edaravone group (dose 6 mg·kg-1·d-1), 16 in each group. At 72 h after reperfusion, each group of mice was scored for neurological impairment. After the end of the experiment, the extent of cerebral infarction in each group of mice was determined by staining with 2,3,5-trichlorophenyl tetrazolium chloride (TTC). Using thiobarbituric acid (TBA) method to measure malondialdehyde (MDA) content in brain tissue, using hydroxylamine method to measure superoxide dismutase (SOD) activity, using 5,5-dithiodinitrobenzoic acid (DTNB) colorimetric assay for glutathione peroxidase (GSH-Px) activity. 【Results】Compared with the sham-operated group, the neurological impairment scores of the model group were significantly increased. The pathological findings were cerebral infarction. The content of MDA in brain tissue was significantly increased, and the activities of SOD and GSH-Px were significantly decreased. The differences were statistically significant. (P<0.01). Compared with the model group, the scores of neurological impairment in the high, medium, and low dose groups of P. oleifera total flavonoids were significantly reduced, the cerebral infarct area was significantly reduced, the content of MDA in the brain tissue was significantly decreased, and the activities of SOD and GSH-Px were significantly increased. , all showed significant dose-effect dependence; among them, the total flavonoids in P. oleracea flavonoids were more effective in the high and middle dose groups (all P<0.05), and there was no statistically significant difference compared with the edaravone group (P). >0.05). 【Conclusion】 The total flavonoids of P. communis has a repairing effect on cerebral ischemia-reperfusion injury in mice, which may be related to its anti-oxidative activity.