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本文采用新生大鼠海马神经元培养技术,以细胞形态学及HSP70免疫阳性细胞表达为指标,首次观察了巴曲酶对缺氧海马神经元损伤的直接保护作用。结果发现:在缺氧前0.5h给予巴曲酶(0.5BU/ml),海马神经细胞存活率以及HSP70免疫阳性细胞率均明显高于缺氧对照组(P<0.01),而在缺氧前24h给予巴曲酶后,二者与缺氧对照组均无明显差别。表明:巴曲酶对缺氧海马神经细胞损伤有直接的保护作用,而且其作用与给药的时机有关。
In this paper, neonatal rat hippocampal neuron culture technology, cell morphology and expression of HSP70-positive cells as an indicator for the first time observed Batroxobin hypoxic hippocampal neuronal damage in a direct protective effect. The results showed that 0.5hU / ml batroxobin (0.5BU / ml), the survival rate of hippocampal neurons and the rate of HSP70 immunoreactive cells in hypoxia group were significantly higher than those in hypoxia control group (P <0.01) 24h before hypoxia given batroxobin, both with hypoxia control group no significant difference. The results showed that batroxobin had a direct protective effect on the injury of hypoxic hippocampal neurons, and its effect was related to the timing of administration.