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目的 对微小隐孢子虫子孢子表面蛋白CP2 3编码区基因gp2 3进行克隆及测序。 方法 提取牛源微小隐孢子虫卵囊基因组DNA ,用聚合酶链反应 (PCR)扩增编码CP2 3的基因 ,然后将其克隆到 pMD1 8 T载体中 ,用双脱氧链末端终止法测DNA序列。 结果与结论 获得了CP2 3的编码区基因 ,克隆出的 gp2 3基因的核苷酸与氨基酸序列长分别为 345bp和 1 1 4aa,有一个开放阅读框 ,与GenBank报道的 gp2 3基因比较 ,同源性分别为 97.3 %与 98 2 % ,在 2 32～ 2 38bp处多了 6bp。
Objective To clone and sequence the gp2 3 gene of CP2 3 coding region of Cryptosporidium parvum. Methods The genomic DNA of Cryptosporidium parvum oocysts was extracted and the gene encoding CP2 3 was amplified by polymerase chain reaction (PCR). The cloned gene was then cloned into pMD18 T vector and the DNA sequence was determined by dideoxy chain termination . RESULTS AND CONCLUSIONS The coding region of CP2 3 was obtained. The nucleotide and amino acid sequence of the cloned gp2 3 gene were 345 bp and 1141 a, respectively. There was an open reading frame, which was compared with the gp23 gene reported by GenBank The source was 97.3% and 98 2% respectively, with an extra 6bp at 2 32 ~ 2 38bp.