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目的探讨放射性粒子125I在动物体内抗人脑胶质瘤的效果及其作用机制。方法建立人胶质瘤的动物模型,瘤内植入放射性粒子125I,观察肿瘤生长情况,在电镜下观察其超微结构的变化,采用TUNEL技术检测肿瘤组织的凋亡情况,流式细胞仪检测Bax/Bcl-2的表达,并应用免疫组化染色检测血管密度和VEGF、bFGF的表达。结果成功建立了人胶质瘤细胞U251的动物模型,植入放射性粒子125I后,胶质瘤生长较对照组明显受到抑制(P<0.05,P<0.01),电镜下可呈现核断裂、染色质边聚等细胞凋亡的形态特征。检测显示:和对照组相比,肿瘤组织的Bax/Bcl-2(1.88±0.47vs1.11±0.52,P<0.01)和组织凋亡率的IHS评分(3.64±0.89vs0.81±0.45,P<0.01)明显增高;肿瘤组织的微血管密度IHS评分(1.40±0.55vs3.23±0.87,P<0.01)、VEGF的IHS评分(1.58±0.55vs4.19±0.45,P<0.01)和bFGF的IHS评分(2.44±0.89vs3.52±0.79,P<0.05)明显降低。结论放射性粒子125I对在体人胶质瘤U251有明确的抑制作用,促进瘤细胞凋亡和降低瘤组织微血管密度可能是其主要作用机制。
Objective To investigate the anti-human glioma effect and its mechanism of radioactive 125I in animals. Methods The animal model of human glioma was established. 125I radioactive particles were implanted in the tumor. The growth of the tumor was observed. The ultrastructure of the tumor was observed under electron microscope. The apoptosis of tumor was detected by TUNEL technique. Flow cytometry Bax / Bcl-2 expression, and immunohistochemical staining of vascular density and VEGF, bFGF expression. Results The animal model of U251 human glioma cells was established successfully. The growth of glioma cells was significantly inhibited compared with the control group (P <0.05, P <0.01) after implantation of 125I radioactive particles, and nuclear fragmentation, chromatin Morphological characteristics of polyglucose apoptosis. The results showed that compared with the control group, the Bax / Bcl-2 (1.88 ± 0.47vs1.11 ± 0.52, P <0.01) and the IHS score of the apoptosis rate of the tumor tissue (3.64 ± 0.89vs0.81 ± 0.45, P (P <0.01), IHS score of VEGF (1.58 ± 0.55vs4.19 ± 0.45, P <0.01) and IHS of bFGF (1.40 ± 0.55vs3.23 ± 0.87, P <0.01) The score (2.44 ± 0.89 vs 3.52 ± 0.79, P <0.05) decreased significantly. Conclusion 125I radioactive particles have a clear inhibitory effect on human glioma U251, promote tumor cell apoptosis and reduce the density of tumor microvessels may be its main mechanism.