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目的 研究心房颤动 (房颤 )患者肌浆网钙释放通道 :ryanodine受体 (ryanodinereceptortype 2 ,RyR2 )及 1,4,5 三磷酸肌醇受体 (1,4,5 trisphosphaterinositolreceptor,IP3 R1)mRNA表达的改变 ,探讨房颤时心肌细胞浆Ca2 + 超负荷的原因。方法 38例风湿性心脏病二尖瓣狭窄接受外科手术者 ,手术时取右心房及右心耳各约 10 0mg ,通过逆转录 聚合酶链反应技术 ,以GAPDH为内参照 ,测量RyR2 及IP3 R1mRNA的变化。结果 房颤患者肌浆网RyR2 和IP3 R1mRNA较窦性心律者上调 ,心房不同部位无差别。地高辛、卡托普利、美托洛尔等药物对RyR2 和IP3 R1mRNA水平表达无明显影响。结论 房颤患者RyR2 和IP3 R1上调 ,说明心肌细胞浆Ca2 + 超负荷与肌浆网钙库的释放增加有关。
Objective To investigate the expression of sarcoplasmic reticulum calcium channel (ryanodine receptor 2, RyR2) and 1,4,5 trisphosphaterinositol receptor (IP3 R1) in patients with atrial fibrillation Of the changes in myocardial fibrinolytic activity of Ca2 + overload causes. Methods Thirty-eight patients with rheumatic mitral stenosis underwent rheumatic mitral stenosis. The right atrial appendage and right atrial appendage were each treated with about 100 mg. Reverse transcription-polymerase chain reaction (RT-PCR) and GAPDH were used as internal reference to measure the expression of RyR2 and IP3R1mRNA Variety. Results Atrial fibrillation patients sarcoma RyR2 and IP3 R1mRNA than sinus rhythm increased, different parts of the atrium without distinction. Digoxin, captopril, metoprolol and other drugs had no significant effect on RyR2 and IP3 R1 mRNA expression. Conclusions Atrial fibrillation patients with RyR2 and IP3 R1 upregulation, indicating that myocardial cytoplasmic Ca2 + overload and sarcoplasmic reticulum calcium store release increased.