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本研究选取小麦品种“沧6001”(抗盐性品种)和“石4185”(盐敏感品种)为试验材料,采用水培方法,对加入蔗糖后两个小麦品种的耐盐性进行比较。结果表明,盐胁迫下,“沧6001”和“石4185”小麦根系生长均受到抑制,“石4185”根系受到抑制的程度大于“沧6001”;蔗糖预处理可以显著缓解盐胁迫对根系生长的抑制作用。蔗糖预处理12 h后,“沧6001”和“石4185”的可溶性糖含量上升,与“石4185”相比,“沧6001”具有更高的可溶性糖水平,但是蔗糖预处理后“石4185”可溶性糖含量的增加量高于“沧6001”。H2DCFDA荧光探针检测和PI荧光染色检测结果表明,蔗糖预处理可有效降低盐胁迫下小麦幼苗体内活性氧累积水平,保护细胞膜的完整性并减小根尖细胞死亡率。蔗糖预处理可以提高小麦幼苗SOD和APX(P<0.05)、CAT活性(P>0.05)。本研究对于理解外源物质对小麦耐盐性影响的生理机制及筛选耐盐性的小麦品种具有指导意义。
In this study, the wheat cultivars Cang 6001 (salt-tolerant varieties) and Shi 4185 (salt-sensitive varieties) were selected as experimental materials, and the salt tolerance of two wheat cultivars Compare. The results showed that under salt stress, the root growth of “Cang 6001” and “Shi 4185” was inhibited, and the degree of “4185” was inhibited more than that of “Cang 6001”; sucrose pretreatment could Significantly alleviate the inhibitory effect of salt stress on root growth. After 12 h of sucrose pretreatment, the soluble sugar content of Cang 6001 and Stone 4185 increased, and Cang 6001 had a higher soluble sugar level than that of Shi 4185, However, after sucrose pretreatment, the increase of soluble sugar content of “Shi 4185” was higher than that of “Cang 6001”. H2DCFDA fluorescence probe detection and PI fluorescence staining results show that sucrose pretreatment can effectively reduce the accumulation of reactive oxygen species in wheat seedlings under salt stress to protect the integrity of the cell membrane and reduce root tip cell death rate. Sucrose pretreatment could increase SOD and APX (P <0.05) and CAT activities of wheat seedlings (P> 0.05). This study is of guiding significance for understanding the physiological mechanisms of exogenous substances affecting the salt tolerance of wheat and for screening salt-tolerant wheat cultivars.