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目的观察谷氨酰胺(GLN)对急性坏死性胰腺炎(ANP)大鼠肠道细菌移位的影响。方法将实验大鼠120只,随机分成对照组、ANP组及GLN组,每组各40只,ANP模型采用结扎胰胆管诱发,GLN组制作ANP后灌入GLN,观察3组肠粘膜组织学和 DNA含量,肠系膜淋巴结,胰腺组织细菌培养及吖啶橙标记菌实验。结果 ANP后 72 h,肠粘膜出现明显损伤, DNA含量下降,肠系膜淋巴结,胰腺组织细菌培养计数明显升高,吖啶橙标记菌移位率明显升高,应用 GLN能明显减轻肠粘膜损伤,降低上述指标。结论GLN有利于维持ANP时肠粘膜屏障功能,减少肠道细菌移位的发生。
Objective To investigate the effect of glutamine (GLN) on intestinal bacterial translocation in rats with acute necrotizing pancreatitis (ANP). Methods 120 experimental rats were randomly divided into control group, ANP group and GLN group, 40 rats in each group, ANP model induced by ligation of pancreaticobiliary duct, GLN group produced ANP and then poured into GLN. The intestinal mucosa histology and DNA content, mesenteric lymph nodes, bacterial culture of pancreatic tissue and acridine orange marker. Results At 72 h after ANP, the intestinal mucosa was obviously damaged and the content of DNA was decreased. The bacterial culture count of mesenteric lymph nodes and pancreas increased significantly, and the translocation rate of acridine orange marked bacteria was significantly increased. GLN could obviously reduce intestinal mucosal damage and decrease The above indicators. Conclusion GLN is beneficial to maintain the function of intestinal mucosal barrier during ANP and reduce the occurrence of intestinal bacterial translocation.