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采用磷酸苯二钠法在520nm波长测定了未成熟、早熟和正常成熟中华绒螯蟹肝胰腺与性腺中的酸性磷酸酶(ACP)和碱性磷酸酶(ALP)。结果表明:(1)各组蟹肝胰腺ACP和ALP活性都显著高于性腺;(2)早熟蟹肝胰腺中ACP活性都显著高于未成熟蟹而低于正常成熟蟹,在各发育阶段没有性别差异,但是,早熟雌蟹和正常成熟雌蟹卵巢中ACP活性都较早熟雄蟹和正常雄蟹精巢低,早熟与正常成熟个体没有差异;(3)早熟雌蟹肝胰腺ALP活性显著高于未成熟和正常成熟雌蟹,而与三组雄蟹处于同一水平,但是,早熟和正常成熟雌雄蟹的性腺中ALP活性没有显著差异。由此得出结论:(1)性腺发育所需营养物质的动员或合成主要与肝胰腺ACP有关;(2)性早熟的发生与肝胰腺中ACP和ALP活性的显著升高有关。
The acid phosphatase (ACP) and alkaline phosphatase (ALP) in hepatopancreas and gonad of immature, precocious and normal mature mitten crab Eriocheir sinensis were determined by diphenyl phosphate method at 520nm wavelength. The results showed that: (1) ACP and ALP activities of crab liver and pancreas in all groups were significantly higher than those in gonads; (2) ACP activity in hepatopancreas of precocious crab was significantly higher than that of immature crab and lower than that of normal mature crab at all developmental stages However, ACP activity in ovary of precocious female and normal mature female crabs was lower than that of premature and normal male cows, and there was no difference in precocious puberty and normal mature individuals. (3) The activity of ALP in hepatopancreas of precocious female crab was significantly higher than that of normal male crab Immature and normal mature female crabs, but with the three groups of male crab at the same level, however, precocious and normal mature male and female crab gonadal ALP activity was no significant difference. The conclusions drawn from this are as follows: (1) Mobilization or synthesis of nutrients required for gonadal development is mainly related to hepatopancreas ACP; (2) The occurrence of precocious puberty is related to the significant increase of ACP and ALP activity in hepatopancreas.