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目的 :测定胰岛细胞移植后移植物内胰岛素量的变化情况。方法 :10周龄纯系雄性Lewis大鼠作为实验的供者与受者 ,300个胰岛分别移植到正常和注射过60mg/kg 链脲霉素(STZ)的Lewis大鼠肾包膜下 ,并观察血糖的变化。通过提取肾脏内胰岛素后 ,再用放射免疫法测定。结果 :在注射STZ并胰岛细胞移植后4周 ,Lewis大鼠的血糖降为 (7.8±3.6 )mmol/L ,接近于正常组血糖值。胰岛移植后8周和12周血糖值有所上升。胰岛移植后3d测得胰岛素量较高 ,以后的14周胰岛素量逐渐减少。结论 :同系胰岛细胞移植后移植物内的胰岛素量会逐渐减少。
OBJECTIVE: To determine the changes of insulin in transplant after islet cell transplantation. Methods: Ten-week-old male Lewis rats were used as experimental donors and recipients. Three hundred and three islets were transplanted into the renal capsule of Lewis rats injected with 60 mg / kg streptozotocin (STZ) Observe the changes in blood sugar. After extracting the kidneys, insulin was measured by radioimmunoassay. Results: Lewis rats’ blood glucose dropped to (7.8 ± 3.6) mmol / L 4 weeks after injection of STZ and islet cell transplantation, which was close to that of the normal group. Blood glucose levels increased at 8 and 12 weeks after islet transplantation. Insulin was measured 3d days after islet transplantation, the amount of insulin gradually decreased after 14 weeks. Conclusion: The amount of insulin in the graft after islet cell transplantation will gradually decrease.