松材线虫(Bursaphelenchus xylophilus)和拟松材线虫(B.mucronatus)被进一步证明是两个不同的种。除表现致病力差异外,形态上具有明显的不同特征,特别是前者交合伞卵圆形,后者平截形;生理学试验表明二者不能交配产生后代;生物化学方面的差异是:酯酶凝胶电泳中松材线虫酯酶带3条,迁移率分别为0.42、0.45、0.49;而拟松材线虫只1条酯酶带,迁移率为0.65。二者脂肪酸总量、不饱和度、短链和长链脂肪酸含量都有明显的差异。 Bursaphelenchus xylophilus and B. mucronatus were further demonstrated to be two different species. In addition to showing the difference in virulence, the morphology has obvious different characteristics, especially the former cross umbrella umbrella oval, the latter truncated; physiological tests show that the two can not produce mating progeny; biochemical differences are: esterase Gel electrophoresis Pine Wood Nematode esterase with 3, the migration rates were 0.42,0.45,0.49; and the loose pine nematode only one esterase band, the mobility was 0.65. Both total fatty acids, unsaturation, short-chain and long-chain fatty acids have significant differences.