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NTPDase(Nucleoside triphosphate-diphosphohydrolase,核苷酸双磷酸酶)在哺乳动物中分布于细胞膜,可以水解细胞外ATP和其他核苷酸,从而调控胞外核苷酸代谢及信号应答.根据水稻NTPD基因序列设计引物,在不同胁迫条件样品中,通过PCR扩增水稻NTPD基因,结果表明水稻NTPD基因表达水平在盐胁迫样品中升高.为进一步分析水稻NTPD基因的功能,构建了该基因的过量表达、RNA干涉载体,通过根癌农杆菌介导转入水稻品种日本晴并获得阳性植株.半定量RT-PCR分析达到过量表达和干涉目的.在盐胁迫条件下,T0代过表达转基因植株表现出一定程度的抗胁迫能力,RNA干涉转基因植株的生长则受到抑制.图7表3参20
NTPDase (Nucleoside triphosphate-diphosphohydrolase) is distributed in the cell membrane in mammals and can hydrolyze extracellular ATP and other nucleotides to regulate extracellular nucleotide metabolism and signal response.According to the NTPD gene sequence The primers were designed to amplify the NTPD gene in rice by PCR under different stress conditions.The results showed that the expression level of NTPD gene in rice increased in salt stress.To further analyze the function of NTPD gene in rice, RNA interference vector was introduced into rice variety Nipponbare through Agrobacterium tumefaciens and positive plants were obtained.The semi-quantitative RT-PCR analysis reached the purpose of overexpression and interference.In the condition of salt stress, T0 generation transgenic plants showed a certain degree Of the anti-stress ability, RNA interference transgenic plant growth was inhibited .Figure 7 Table 3 参 20