RNAi是近几年发展起来的抑制基因表达的新技术。它通过dsRNA介导的特异性降解相应序列的mRNA ,从而导致转录后的基因沉默。目前已在线虫、果蝇、锥虫、真菌、小鼠及植物等生物中建立RNAi技术。其可以作为一种简单有效的代替基因敲除的分子遗传的研究工具 ,在后基因组时代寄生虫的基因功能研究和药物开发中具有广阔的前景 RNAi is a new technology that has been developed in recent years to inhibit gene expression. It specifically degrades mRNAs of the corresponding sequences through dsRNA-mediated silencing, leading to post-transcriptional gene silencing. At present, RNAi technology has been established in organisms such as nematodes, fruit flies, trypanosomes, fungi, mice and plants. It can be used as a simple and effective molecular genetic research tool to replace gene knockout, and has broad prospects in the research of gene function and drug development of parasites in the post-genome era