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利用同位素标记的抗人活化血小板单克隆抗体,经放射免疫法来评估14例支气管哮喘发作时体内血小板的活化程度,结果为:(1)哮喘发作时血小板α-颗粒膜蛋白(GMP-140)分子数在质膜上表达显著增高(P<0.001),血浆内GMP-140的分子数也显著升高(P<0.001),而血小板膜糖蛋白Ⅰ_b及Ⅲ_a的分子数无显著改变(P>0.05);(2)血浆内血栓素B_2(TXB_2)的浓度呈显著升高,TXB_2与6-酮-PGF_(1a)的比值显著增高(P<0.01);(3)血小板α-颗粒释放产物β-血小板球蛋白(β-TG)及血小板因子4(PF_4)的血浆浓度均显著升高;(4)血小板数量明显下降。结果表明哮喘发作时体内血小板的活化程度显著增高,为进一步研究血小板活化在哮喘发病中的意义提供了依据。
The use of isotope labeled anti-human activated platelet monoclonal antibody, radioimmunoassay to evaluate the 14 cases of bronchial asthma attack in vivo platelet activation, the results are as follows: (1) asthma attack platelet α-granule membrane protein (GMP-140) (P <0.001). The number of GMP-140 in plasma was also significantly increased (P <0.001), whereas the numbers of platelet glycoproteins Ⅰ_b and Ⅲa did not change significantly (P> 0.05). (2) The concentration of thromboxane B 2 (TXB 2) in plasma increased significantly and the ratio of TXB 2 to 6-keto-PGF 1a increased significantly (P <0.01). (3) The release of platelet α- Plasma levels of β-thromboglobulin (β-TG) and platelet factor 4 (PF_4) were significantly increased; (4) Platelet count decreased significantly. The results show that the degree of activation of platelets in asthma attack significantly increased, to provide a basis for further study of the significance of platelet activation in the pathogenesis of asthma.