论文部分内容阅读
采用通用引物PCR(UPPCR)、PCR RFLP、PCR SSCP技术 ,研究快速鉴别鱼病病原菌的分子生物学诊断技术。结果发现 ,采用细菌 16SrRNA基因保守区特异性引物 ,以嗜水气单胞菌、鲁克氏耶尔森菌、鳗弧菌、柱状曲挠杆菌、乙型链球菌、荧光假单胞菌等部分常见鱼病病原菌为对象 ,可以建立一种UPPCR技术。该技术能在保证实验条件不变的基础上 ,检出上述所有细菌 ,并还可检出大肠杆菌和双歧杆菌等非鱼病病原菌。并且认为 ,该法与SSCP配合即采用UPPCR SSCP技术能较好地鉴别被检菌而用于鱼病病原菌的快速诊断。
The universal primers PCR (UPPCR), PCR RFLP and PCR SSCP techniques were used to study the molecular biological diagnostic techniques for rapid identification of pathogenic bacteria of fish diseases. The results showed that the use of bacterial 16SrRNA gene-specific primers, to Aeromonas hydrophila, Yersinia pestis, Vibrio anguillarum, Campylobacter, Streptococcus, Pseudomonas fluorescens and other parts Common fish disease pathogens as a target, you can establish a UPPCR technology. The technology can ensure that the experimental conditions remain unchanged, based on the detection of all the above bacteria, and also detect E. coli and bifidobacteria non-fish pathogens. And that the law and the SSCP UPPCR SSCP technology that can be used to better identify the bacteria were used for rapid diagnosis of fish pathogens.