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目的:提取和纯化Sa抗原。方法:应用DE52离子层析法。结果:Sa抗原的分子量是50KD,存在于0.20mol/LNaclTBS洗脱组分中,在-20℃保存期为120天。结论:分离和纯化的Sa抗原可应用于免疫印迹检测抗Sa抗体。
Objective: To extract and purify Sa antigen. Methods: DE52 ion chromatography was used. Results: The molecular weight of the Sa antigen was 50KD and was present in the 0.20mol / L NaCl wash buffer at 120 ° C for 120 days. Conclusion: The isolated and purified Sa antigen can be used to detect anti-Sa antibody by immunoblotting.