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目的观察坐骨神经分支选择性损伤(SNI)动物制作型模后不同时间点L4~6背根神经节(DRG)水平P2X3mRNA的表达情况,探讨外周P2X3mRNA在神经病理性痛模型不同阶段中的作用。方法 54只健康雄性SD大鼠完全随机分为空白对照(control)组、假手术(sham surgery)组和手术(surgery)组。通过结扎腓总神经及切断胫神经,保留腓肠神经的方法建立SNI模型。动态观察造模前、造模后1d、3d、7d和14d双侧足跖机械痛阈;Real-time PCR法检测患侧造模后3d、7d和14d L4~6 DRG水平P2X3mRNA表达情况。结果模型制作后各时间点,surgery组大鼠患侧足跖痛阈明显降低(P<0.05),sham surgery组大鼠与control组比较差异无显著性(P>0.05);各组大鼠健侧足跖痛阈于模型制作后各时间点均无显著变化(P>0.05)。模型制作后3d、7d,surgery组大鼠L4、L5、L6 DRG水平P2X3mRNA表达均有不同程度的提高(P<0.05);而模型制作后14d,surgery组大鼠L5、L6DRG水平P2X3mRNA表达明显减少(P<0.05),L4 DRG水平P2X3mRNA表达仍显著多于sham surgery组(P<0.05)。模型制作后各时间点、各DRG水平,sham surgery组和surgery组大鼠P2X3mRNA表达差异均无显著性(P>0.05)。结论外周P2X3mRNA参与SNI模型疼痛的产生和维持,且其在不同阶段发挥的作用不同。
Objective To observe the expression of P2X3 mRNA in DRG of L4 ~ 6 DRG at different time points after sciatic nerve selective injury (SNI), and to explore the role of peripheral P2X3 mRNA in different stages of neuropathic pain model. Methods Fifty-four healthy male Sprague-Dawley rats were randomly divided into blank control group, sham surgery group and surgery group. The SNI model was established by ligating the common peroneal nerve and cutting off the tibial nerve and preserving the sural nerve. The mechanical pain threshold of bilateral foot plantations was observed dynamically on the 1st day, the 3rd day, the 7th day and the 14th day after modeling. The expression of P2X3 mRNA at L4 ~ 6 DRG level was detected by Real-time PCR at 3d, 7d and 14d. Results At each time point after the model was established, the pain threshold of the affected side of the surgery group was significantly decreased (P <0.05), while there was no significant difference between sham surgery group and control group (P> 0.05) Lateral plantar pain threshold at the time point after model making no significant change (P> 0.05). The expression of P2X3mRNA in L4, L5 and L6 DRG groups increased to some extent at 3d, 7d after surgery (P <0.05), but the expression of P2X3mRNA in L5 and L6DRG group decreased significantly (P <0.05). P2X3 mRNA expression in L4 DRG group was still significantly higher than sham surgery group (P <0.05). There were no significant differences in the expression of P2X3 mRNA between the sham surgery group and the surgery group at each time point after DRG intervention (P> 0.05). Conclusion Peripheral P2X3 mRNA is involved in the production and maintenance of pain in SNI model, and its role in different stages is different.