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目的建立一种同时测定水果中9种杀菌剂残留的超高效液相色谱-串联质谱分析方法。方法样品经乙腈超声提取,离心后取上清液过固相萃取柱净化,通过ACQUITY UPLC HSS T3色谱柱分离,以乙腈-0.05%甲酸水作为流动相进行梯度洗脱,采用多反应监测(MRM)模式,以外标法定量。结果 9种杀菌剂的质量浓度为20 ng/ml~1 000 ng/ml时,线性关系良好,相关系数(r)≥0.995 0,检出限为0.4μg/kg~2.0μg/kg。方法的平均回收率为83.2%~101.0%,相对标准偏差(RSD)为2.3%~6.6%。结论该方法具有灵敏度高、样品前处理简单、分析时间短等优点,可满足水果中9种常见杀菌剂的检测要求。
Objective To establish a method for the simultaneous determination of nine fungicides in fruits by ultra performance liquid chromatography-tandem mass spectrometry. Methods The samples were extracted by acetonitrile and centrifuged. The supernatants were purified by solid phase extraction (SPE) and separated on an ACQUITY UPLC HSS T3 column. The mobile phases were eluted with acetonitrile - 0.05% formic acid as mobile phase. Multiple reaction monitoring ) Model, the amount of external standard method. Results The linearity of the nine fungicides was 20 ng / ml ~ 1 000 ng / ml with the correlation coefficient (r) ≥0.995 0 and the detection limit of 0.4 μg / kg ~ 2.0 μg / kg. The average recoveries of the method ranged from 83.2% to 101.0%, and the relative standard deviations (RSDs) ranged from 2.3% to 6.6%. Conclusion The method has the advantages of high sensitivity, simple sample preparation, short analysis time and so on, which can meet the detection requirements of nine kinds of common fungicides in fruit.