论文部分内容阅读
背景:谷胱甘肽S-转移酶具有清除活性氧、提高机体抗氧化能力的作用。目的:分析谷胱甘肽S-转移酶P1～105基因多态性与高原环境下人体运动能力的关联性。设计、时间及地点:对照比较分析,于2007年在解放军体育进修学院完成。对象:实验组86名高原登山运动员为在高原环境下具有较强的作业能力的特定人群,对照组为解放军体育学院随机选取的90名健康学员。方法:采集86名高原登山运动员及90名健康学员的血样,提取基因组DNA,采用PCR-RFLP技术检测谷胱甘肽S-转移酶P1～105基因多态性,分别比较高原登山运动员与平原汉族对照人群间谷胱甘肽S-转移酶P1～105等位基因和基因型的分布。主要观察指标:谷胱甘肽S-转移酶P1～105基因型的分布。结果:共检测到谷胱甘肽S-转移酶P1～105基因3种基因型:变异杂合型(A/G)、变异纯合型(G/G)、野生基因型(A/A)。实验组谷胱甘肽S-转移酶P1～105的G等位基因、变异基因型(A/G+G/G)的频率皆明显低于对照组(P<0.01)。以变异基因型作为暴露因素,求得OR=2.19,95%CI=1.16～4.13,提示在高原环境下,与具有谷胱甘肽S-转移酶P1～105野生基因型人的运功能力比较,具有谷胱甘肽S-转移酶P1～105变异基因型人的运动能力下降了1.19倍。结论:谷胱甘肽S-转移酶P1～105基因多态性与高原环境下人体运动能力相关,野生基因型表现出明显的运动优势。
Background: Glutathione S-transferase has the function of scavenging reactive oxygen species and enhancing the body’s antioxidant capacity. OBJECTIVE: To analyze the association of glutathione S-transferase P1 ~ 105 gene polymorphisms with the exercise capacity in the plateau environment. DESIGN, TIME AND SETTING: Comparative comparative analysis was completed in 2007 in the People’s Liberation Army Physical Training Institute. PARTICIPANTS: The experimental group of 86 plateau mountaineers was a specific population with strong working ability in the plateau environment. The control group consisted of 90 randomly selected healthy students from the PLA Sports Academy. Methods: The blood samples of 86 alpine climbers and 90 healthy students were collected and genomic DNA was extracted. The polymorphisms of glutathione S-transferase P1 ~ 105 were detected by PCR-RFLP. The differences between plateau mountaineers and plain Han Distribution of glutathione S-transferase P1 ~ 105 alleles and genotypes in control population. MAIN OUTCOME MEASURES: Distribution of glutathione S-transferase P1 ~ 105 genotypes. RESULTS: Three genotypes of glutathione S-transferase P1105 gene were detected: heterozygous (A / G), homozygous (G / G), wild type (A / A) . The frequencies of G allele and variant genotype (A / G + G / G) of glutathione S-transferase P1 ~ 105 in the experimental group were significantly lower than those in the control group (P <0.01). The variation genotype was taken as the exposure factor, and OR = 2.19,95% CI = 1.16 ~ 4.13 was obtained, which suggested that compared with those with wild-type glutathione S-transferase P1105 wild type, , With glutathione S-transferase P1 ~ 105 variant genotypes decreased 1.19 times the motor ability. CONCLUSION: The polymorphisms of glutathione S-transferase P1105 gene are related to the exercise capacity of human body in the plateau environment, and the wild type genotypes show obvious motor advantage.