一、母种制法 1、配制母种培养基 最常用的是PDA斜面培养基,又称马铃薯葡萄糖培养基,配制方法是:取无病害马铃薯(土豆),洗净去皮,切成薄片,称取200克,加水1000毫升煮沸,直至马铃薯酥而不化为度,用6层纱布过滤,滤液用清水补足至1000毫升,再加琼脂及葡萄糖各20克,继续加热至溶化,趁热分装至试管中,每管8毫升左右。试管用180×18毫米规格的,用前充分 First, the mother of the system of law 1, the preparation of the mother medium is the most commonly used PDA slant medium, also known as potato dextrose medium, the preparation method is: take disease-free potato (potato), wash the skin, cut into thin slices, Weigh 200 grams, add water 1000 ml boil until potato crisp and not degree, with six layers of gauze filter, the filtrate with water to make up to 1000 ml, plus agar and glucose 20 grams, continue heating to melt, while hot Into the test tube, each tube about 8 ml. Test tube with 180 × 18 mm size, fully used before