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本研究旨在研究解淀粉芽孢杆菌(Bacillusamyloliquefaciens,BA)对环磷酰胺(cyclophosphamide,CY)介导的免疫抑制肉鸡生长性能和肝脏抗氧化功能的影响。144只1日龄AA雄性肉仔鸡被随机分为3组,即对照组(CON),环磷酰胺处理组(CY)和环磷酰胺-解淀粉芽孢杆菌组(CY-BA),每组6重复,每重复8只鸡。CON组和CY组饲喂基础日粮,CY-BA组饲喂添加1.08×1010CFU/kg BA的试验日粮。于16、17和18日龄对CY组与CY-BA组肉鸡肌肉注射80mg/kg体重的CY,CON组肌肉注射等量生理盐水。试验期为21天。结果表明:与CON组相比,CY刺激显著降低了CY组肉鸡1~21日龄体增重、肝脏指数、肝脏总抗氧化能力(totalantioxidativecapacity,TAOC)、谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)活力和谷胱甘肽还原酶(glutathionereductase,GR)活力(P<0.05),显著升高了肝脏丙二醛(malondialdehyde,MDA)含量(P<0.05);Real-time PCR检测也发现,CY处理显著降低了CY组肉鸡肝脏核转录相关因子2(NF-E2-relatedfactor2,Nrf2)和谷胱甘肽过氧化物酶1(glutathione peroxidase1,Gpx1)的m RNA表达水平(P<0.05)。与CY组相比,日粮添加BA显著提高了CY-BA组肉鸡21日龄时肝脏T-AOC水平和GSH-Px活力,降低了肝脏MDA水平(P<0.05)。上述指标在CON与CY-BA组间并无明显差异(P>0.05)。另外,CY-BA组肝脏GSH含量较CON或CY组均显著升高(P<0.05)。然而,日粮添加BA并未显著影响CY刺激肉鸡体增重、肝脏指数、肝脏Nrf2和Gpx1m RNA水平(P>0.05)。综合本文结果表明,CY诱导的免疫抑制损伤了肉鸡生长和肝脏的抗氧化功能,并引起了肉鸡肝脏脂质过氧化程度升高。日粮添加BA具有改善CY介导的免疫抑制肉鸡肝脏抗氧化功能和缓解肝脏氧化损作用。
The aim of this study was to investigate the effects of Bacillus amyloliquefaciens (BA) on the growth performance and the anti-oxidative function of liver in immunosuppressed broilers induced by cyclophosphamide (CY). One hundred and forty four one-day-old AA male broilers were randomly divided into three groups: control group (CON), cyclophosphamide-treated group (CY) and cyclophosphamide-Bacillus amyloliquefaciens group (CY-BA) Repeat, every 8 chickens. The basal diet was fed to the CON and CY groups, while the CY-BA fed to the experimental diet supplemented with 1.08 × 1010 CFU / kg BA. At 16, 17 and 18 days of age, CY and CY-BA broilers were injected intramuscularly with 80 mg / kg body weight of CY. Con groups were intramuscularly injected with normal saline. The test period is 21 days. The results showed that compared with the CON group, CY stimulation significantly reduced body weight gain, liver index, total active antioxidant capacity (TAOC), glutathioneperoxidase , GSH-Px) and glutathionereductase (GR) activity (P <0.05), significantly increased hepatic malondialdehyde (MDA) It was also found that CY treatment significantly reduced the m RNA expression of hepatic nuclear transcription factor 2 (Nrf2) and glutathione peroxidase 1 (Gpx1) in CY group (P < 0.05). Dietary supplementation of BA significantly increased liver T-AOC and GSH-Px activities, and decreased liver MDA levels (P <0.05) at 21 days of CY-BA compared with CY. The above indexes had no significant difference between CON and CY-BA groups (P> 0.05). In addition, the liver GSH levels of CY-BA group were significantly higher than that of CON or CY group (P <0.05). However, dietary supplementation of BA did not significantly affect the body weight gain, liver index, hepatic Nrf2 and Gpx1m RNA levels stimulated by CY (P> 0.05). Based on the results of the present study, CY induced immunosuppression impaired broiler growth and hepatic antioxidant activity, and caused an increase in liver lipid peroxidation in broiler chickens. The dietary supplementation with BA can improve the anti-oxidative function of liver mediated by CY and reduce the liver oxidative damage.