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目的：探索某些眼眶良性淋巴增生病向恶性淋巴瘤转变的实质。方法：应用聚合酶链反应（ＰＣＲ）对１８例病理组织学诊断为眼眶良性淋巴增生病的石蜡切片进行免疫球蛋白重链（ＩｇＨ）基因扩增。结果：３例良性淋巴增生病见ＩｇＨ基因一致性重排，电泳产物见一明显的扩增带（１００～１２０ｂｐ），其余１５例未见扩增产物或呈多克隆带。结论：某些良性淋巴增生病的病例存在病理组织学和免疫组织化学不能检测到的微小单克隆性病变。它提示临床为良性过程的病变可能包含着隐匿的单克隆Ｂ细胞亚群。ＰＣＲ扩增ＩｇＨ基因序列能更客观和敏感地鉴别眼眶淋巴增生病的克隆性质。
Objective: To explore the essence of some benign orphan hyperplasia or malignant lymphoma. Methods: Immuno-globulin heavy chain (IgH) gene was amplified by polymerase chain reaction (PCR) in 18 paraffin sections of pathologically diagnosed orbital benign lymphoproliferative disease. Results: The consistent rearrangement of IgH gene was observed in 3 cases of benign lymphoproliferative diseases. The products of the electrophoresis showed a clear amplification band (100-120 bp), and the other 15 cases showed no amplification products or polyclonal bands. Conclusions: In some cases of benign lymphoproliferative diseases, there are histopathological and microscopic monoclonal lesions that can not be detected by immunohistochemistry. It suggests that clinically benign lesions may harbor an insidious subset of monoclonal B cells. PCR amplification of IgH gene sequence can more objectively and sensitively identify the clonal nature of orbital lymphoproliferative disease.