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目的与方法造影剂微泡浓度、大小是影响心肌声学显影最重要因素。本研究采用２×２×４析因分析法分析不同超声照射条件对造影剂微泡浓度及直径的影响，即声波频率、能量以及照射时间对微泡浓度、大小的单独及交互作用。为临床行静脉心肌声学造影检查时选择适宜的超声照射条件提供参考。结果能量越大、照射时间越长，微泡破坏越多，平均直径越小：照射频率对微泡浓度影响不大，但影响微泡大小，频率越高，微泡越小。结论为减少超声照射对微泡的破坏，提高心肌声学造影效果，应尽可能选用低能量、低频率超声波，并减少不必要的照射时间。
Aims and methods Contrast microbubble concentration, size is the most important factor affecting myocardial acoustic imaging. In this study, 2 × 2 × 4 factorial analysis was used to analyze the effect of different ultrasound irradiation conditions on the concentration and diameter of contrast medium microbubbles, ie, the frequency and energy of the sound waves and the irradiation time on the concentration and size of the microbubbles. For the clinical venous myocardial contrast echocardiography to choose the appropriate ultrasound irradiation conditions provide a reference. Results The larger the energy, the longer the irradiation time, the more the microbubbles destroyed, and the smaller the average diameter. The frequency of irradiation had little effect on the concentration of microbubbles but affected the size of microbubbles. The higher the frequency, the smaller the microbubbles. Conclusion To reduce the destruction of microbubbles by ultrasound irradiation and improve the myocardial contrast echocardiography, we should use low-energy, low-frequency ultrasound as much as possible and reduce unnecessary irradiation time.