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目的:研究番茄红素对鱼藤酮诱导PC12细胞线粒体损伤的保护作用。方法:采用鱼藤酮(0.5μmol·L-1)诱导PC12细胞损伤模型,给予番茄红素(3,10,30μmol·L-1)预处理2 h后,加入鱼藤酮,使终浓度达到0.5μmol·L-1,培养24 h后,CCK-8法检测细胞存活率,倒置相差显微镜观察细胞形态改变、透射电镜观察细胞线粒体超微结构,罗丹明123染色检测线粒体膜电位,以观察番茄红素的保护作用。结果:CCK-8结果显示与正常对照组比较,模型组细胞存活率下降为70.34%±2.81%(P<0.05),与模型组比较,番茄红素(3,10,30μmol·L-1)预处理后其细胞存活率分别提高到83.09%±3.15%,87.24%±2.15%,89.17%±2.26%(P<0.05);倒置相差显微镜观察显示鱼藤酮可导致PC12细胞凋亡,经番茄红素处理后,模型细胞凋亡情况明显改善;透射电镜观察显示鱼藤酮可导致PC12细胞线粒体超微结构发生改变,经番茄红素处理后,模型细胞凋亡情况明显改善;流式细胞仪检测结果显示与正常对照组比较,模型组细胞线粒体膜电位平均荧光强度下降为151.63±12.25(P<0.05),经番茄红素(3,10,30μmol·L-1)预处理后线粒体膜电位平均荧光强度分别提高到202.24±26.28,226.21±9.71,238.83±10.29(P<0.05)。结论:番茄红素对鱼藤酮诱导的PC12细胞线粒体损伤有保护作用。
Objective: To study the protective effect of lycopene on rotenone-induced mitochondrial damage in PC12 cells. Methods: PC12 cells were induced by rotenone (0.5μmol·L-1), and pretreated with lycopene (3, 10, 30μmol·L-1) for 2 hours. Rotenone was added to make the final concentration reach 0.5μmol·L The cell viability was detected by CCK-8 assay after 24 h incubation. Cell morphology was observed by inverted phase contrast microscope. The ultrastructure of mitochondria was observed by transmission electron microscopy. Mitochondrial membrane potential was detected by rhodamine 123 staining to observe the protection of lycopene effect. Results: Compared with the normal control group, the cell viability in model group decreased by 70.34% ± 2.81% (P <0.05). Compared with the model group, the lycopene (3, 10, 30μmol·L- After pretreatment, the cell viability increased to 83.09% ± 3.15%, 87.24% ± 2.15% and 89.17% ± 2.26% (P <0.05), respectively. The inverted phase contrast microscope showed that rotenone could induce the apoptosis of PC12 cells. Lycopene Transmission electron microscopy showed that rotenone could change the ultrastructure of mitochondria in PC12 cells. After lycopene treatment, the apoptosis of model cells was significantly improved. The results of flow cytometry The average fluorescence intensity of mitochondrial membrane potential in model group decreased to 151.63 ± 12.25 (P <0.05). The average fluorescence intensity of mitochondrial membrane potential after pretreatment with lycopene (3, 10, 30μmol·L-1) Increased to 202.24 ± 26.28, 226.21 ± 9.71, 238.83 ± 10.29 (P <0.05). Conclusion: Lycopene has a protective effect on rotenone-induced mitochondrial damage in PC12 cells.